Table 1.
Distribution of two-pore-domain K+ channel mRNA expression in the CNS
| TASK-1 | TASK-3 | TREK-1 | TREK-2 | TRAAK | TWIK-1 | |
|---|---|---|---|---|---|---|
| Medulla and spinal cord | ||||||
| Dorsal horn/spinal trigeminal nucleus | ++ | ++ | – | +/– | +/– | +/– |
| Nucleus of the solitary tract | ++ | ++++ | ++/+++ | – | – | – |
| Cochlear nuclei | ++ | +/++ | – | – | – | ++ |
| Somatic motor neurons | ++++ | +++++ | +/– | – | – | – |
| Reticular fields | ++ | +/+++ | – | ++/– | – | +/++ |
| Brainstem aminergic nuclei | ||||||
| Raphe nuclei | +/++ | ++++ | +/++ | +/– | – | – |
| Substantia nigra | – | – | – | – | – | +/– |
| Locus ceruleus | +++ | +++++ | – | – | – | – |
| Midbrain and cerebellum | ||||||
| Superior/inferior colliculus | +/– | +/+++ | + | – | – | +/– |
| Periaqueductal gray | +/– | ++/++++ | + | – | – | +/– |
| Red nucleus | +/– | +++ | – | + | – | ++ |
| Pontine nuclei | +++ | +++ | – | + | +/– | ++ |
| Interpeduncular nucleus | +++ | +++ | ++ | – | – | – |
| Vestibular nuclei | +/– | + | – | – | – | +/++ |
| Cerebellar granule cell layer | ++++ | +++++ | + | ++++ | + | ++++ |
| Hypothalamus | ||||||
| Preoptic nuclei | +/++ | +++ | ++/+++ | – | + | – |
| Suprachiasmatic nucleus | +/++ | +++++ | – | – | +/++ | – |
| Supraoptic nucleus | +/++ | + | + | + | + | – |
| Lateral hypothalamic area | +/++ | +/++ | +/++ | – | – | – |
| Paraventricular nucleus | + | ++ | + | – | +/– | – |
| Arcuate nucleus | ++/+++ | ++++ | +++ | – | +/– | – |
| Dorsomedial/ventromedial nuclei | +/++ | ++/+++++ | +/++ | – | +/++ | – |
| Mammillary nuclei | +/++ | +++/++++ | +/– | +/– | + | – |
| Thalamus | ||||||
| Principal relay nuclei | +/++ | ++/+++ | – | – | +/++ | +/++ |
| Intralaminar nuclei | +/++ | +/++ | +/– | +/– | +/++ | +/++ |
| Reticular nucleus | +/++ | +/– | +/++ | + | – | +++/++++ |
| Habenula | + | +/++ | – | +/– | – | +/++++ |
| Basal forebrain | ||||||
| Striatum/accumbens | – | –/++++ | +++/++++ | – | + | + |
| Olfactory tubercle | +/++ | –/++++ | +++/++++ | + | + | + |
| Globus pallidus | +/++ | +/++ | – | – | – | +/++ |
| Subthalamic nucleus | ++ | +++ | – | – | +/– | ++ |
| Bed nucleus of the stria terminalis | +/++ | ++++ | +/++ | – | + | – |
| Septum/diagonal band | +/++ | +/++ | – | – | – | – |
| Amygdala | ||||||
| Cortical nuclei | +/++ | +/+++ | +/++ | +/++ | +/++ | +/+++ |
| Nucleus of the lateral olfactory tract | +/++ | ++++ | +++ | – | ++ | + |
| Central nucleus | – | + | + | – | – | – |
| Medial nucleus | +/++ | ++/++++ | +/++ | +/++ | + | + |
| Lateral nucleus | – | + | +/– | +/++ | +/++ | +/++ |
| Basolateral/basomedial nuclei | – | + | – | – | +/– | + |
| Hippocampus | ||||||
| Pyramidal cell layer, CA1 | +/++ | ++ | +/++ | –/+ | ++ | –/+ |
| Pyramidal cell layer, CA2 | +/++ | + | ++/+++ | –/+ | ++ | +/++ |
| Pyramidal cell layer, CA3 | +/++ | –/++ | – | +/++ | ++ | +/++ |
| Granule cell layer, dentate gyrus | +/++ | +/++++ | +/++ | + | ++ | ++ |
| Indusium griseum | +/– | + | +++ | – | ++ | + |
| Cortex | ||||||
| Neocortex, layers II/III | + | ++/+++ | – | –/+ | ++ | ++/+++ |
| Neocortex, layer IV | +/++ | ++ | ++/+++ | –/+ | ++/+++ | ++ |
| Neocortex, layer V | +/++ | ++ | + | –/+ | ++ | ++ |
| Neocortex, layer VI | +/++ | ++ | + | –/+ | ++ | ++ |
| Piriform cortex | +/++ | ++++ | ++/++++ | +/++ | +++/++++ | +++/++++ |
| Claustrum | +/++ | ++/+++ | – | – | +/++ | +/++ |
| Olfactory system | ||||||
| Olfactory bulb, glomerular layer | +/++ | +/++ | – | – | – | – |
| Olfactory bulb, granule cell layer | ++/+++ | ++/+++ | +/++ | +/++ | + | +/++ |
| Anterior olfactory nucleus | +/++ | ++/+++ | –/+++ | – | +/++ | +/++ |
-
In situ hybridization signals were determined based on relative optical density of film autoradiograms, as well as silver grain density over cells from emulsion-dipped sections. +++++, Highest levels of labeling; ++++, very high; +++, high; ++, moderate; +, low levels; –, signal very close to background. Multiple designations for a single region indicates heterogeneity of labeling in that area. See Results for details.
