Expression of mGlu1a and mGlu5 receptors in Purkinje, granule, and glial cells in cerebellar cultures.A shows the expression of the mGlu1a receptor in granule (arrowheads) and Purkinje cells (arrow) in a representative culture at 6 DIV. The receptor is expressed in both cell types at any developmental stages since at least 3 DIV. Note the absence of staining in glial cells (asterisks). A double immunostaining for mGlu5 receptors (green) and calbindin (red) is shown in B andC, respectively. Note that mGlu5 receptors are expressed in granule cells (arrowheads) but not in Purkinje cells (arrow). A double immunostaining for GFAP (yellow) and mGlu5 antibodies (DAB staining) is shown in D and E. Note the presence of mGlu5 receptors in glial processes. Scale bars: A, 40 μm; B–E, 50 μm.