Effects of mGlu1 and mGlu5 antisense treatment on the morphology of Purkinje cells. A substantial reduction of mGlu1a immunostaining is shown in the Purkinje cells of animals treated with mGlu1 antisense oligonucleotides (D,E), as compared with animals injected with saline (A, B) or with animals treated with mGlu5 antisense oligonucleotides (G,H). The effect of the treatment on the morphology of Purkinje cells is shown in C, F, andI, in which cells are revealed by calbindin immunostaining. Scale bars: A, D,G, 1 mm; B–I, 20 μm.