Treatment with mGlu5 antisense oligonucleotides reduces cell density in the internal granular layer (IGL) in neonate rats. A, Western blot analysis of mGlu5 receptors in hemicerebella from neonate rats treated with mGlu5 antisense oligonucleotides (ASm5) or with a scrambled oligonucleotide (SCR). Note the reduction in mGlu5 receptor expression in four of the five animals treated with antisense oligonucleotides. An example of the reduction in granule cell density in animals treated with antisense oligonucleotides as compared with animals treated with the scrambled oligonucleotides is shown is C and B, respectively (seearrows).