In situ hybridization of brains from 5-month-old TgKO mouse (A, C,E) and corresponding wild-type mouse (B,D, F) with DIG-labeled Cdk5 cRNA antisense probe. A, The signal was revealed in perikarya of the neurons throughout the cerebral cortex. C, A high level of Cdk5 expression was observed in hippocampal pyramidal cells and granule cells in dentate gyrus. E, The somata of the Purkinje cells were highly stained, and the granule cells and the molecular cells were stained moderately. Scale bar: A,B, 175 μm; C, D, 200 μm; E, F, 34 μm.