NT neurotransmission is altered in isolation-reared animals. a, NT receptor binding and NT mRNA expression in the shell of the nucleus accumbens in isolation-reared and socially reared animals. Deficits in PPI in isolation-reared animals were confirmed immediately before killing the animals. Data are expressed as mean ± SEM NT receptor binding (in nanocuries per milligram of tissue) and mean ± SEM NT mRNA expression (in nanocuries per milligram of tissue). *p < 0.05 compared with socially reared rats within a brain region (n = 5–7 females).b, NT mRNA in situ hybridization in the nucleus accumbens of socially reared and isolation-reared animals.c, Effect of quetiapine on NT mRNA expression in the nucleus accumbens shell. Data are expressed as mean ± SEM NT mRNA expression (in nanocuries per milligram of tissue). *p < 0.05 compared with socially reared animals within a treatment group. +p < 0.05 compared with vehicle treated isolation-reared animals (n = 5 or 6 females).