Regulation of Neurotransmitter Vesicles by the Homeodomain Protein UNC-4 and Its Transcriptional Corepressor UNC-37/Groucho inCaenorhabditis elegans Cholinergic Motor Neurons

Table 1.

UNC-17 and ChAT antibody staining

% UNC-17 staining % ChAT staining
DA/VA SAB1-a VC DA/VA SAB1-a VC
wild type 72  (10) 100 98 76  (10) 79 72
unc-4(wd1) 10  (16) 56 3 4  (12) 29 2
unc-4(e26) 8  (14) 75 0 6  (12) 41 0
unc-37(e262) 5  (27) 88 2 7  (14) 62 0
unc-4(e120) 7  (13) 58 0 5  (12) 44 0
unc-4(e120);unc-37(wd17) 14  (16) — — — — —
unc-4(e2321) 7  (13) 79 0 5  (9) 54 2
unc-4(e2321);unc-37(wd17) 48  (18) — — — — —
unc-37(wd17) 96  (0) — — — — —
  • %, Percentage of neurons that show wild-type levels of antibody staining. Numbers in parentheses refer to DA/VA motor neurons showing reduced or intermediate levels of antibody staining (see Materials and Methods). UNC-17 and ChAT expression in DA and VA motor neurons was scored as described below (see Fig. 2). Neurons were scored for DAs/VAs (126 ≤ n ≤ 416). VC vulval varicosities (n = 60) and SAB-containing processes (n = 120) were scored (see Fig. 8).

  • −, Not scored.

  • ↵F1-a  SAB-containing head sublateral nerve cords also include axonal processes from SAA neurons (White et al., 1986).

  1. The Journal of Neuroscience vol. 21 no. 6 2001-2014
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