CEP-1347 blocks c-Jun phosphorylation after NGF deprivation. Neurons were maintained in NGF (A, D), deprived of NGF (B, E), or deprived of NGF in the presence of CEP-1347 (C, F) for 8 hr. Nuclei were stained with Hoechst 33258 (A–C), and immunohistochemistry was performed in parallel with an antibody specific for c-Jun that was phosphorylated at Serine residue 63 (D–F). Robust staining was seen in cultures deprived of NGF, but not in NGF-maintained cultures or NGF-deprived CEP-1347-treated cultures. Scale bar, 10 μm.