Adenovirus-mediated TGF-β1 overexpression activates Erk1/2 and Rsk1 and increases phosphorylation of Bad in the striatum of nonischemic mice. A, Representative blots for P-Erk1/2, P-Rsk1 (Thr³⁶⁰/Ser³⁶⁴), P-Bad (Ser¹¹²), Bad, and α-tubulin. Mice were subjected to intracerebral injection of either AdRSVlacZ or AdRSVahTGF-β1. The striatum tissues were isolated, and proteins were extracted at different time points after injection. Sixty micrograms of total protein were loaded to each lane for Western blot. Blots were representative of three animals at each time point. B. Semiquantification of blots. The ratio of the IOD of P-Erk1, 2, P-Rsk1, P-Bad, and Bad signals to α-tubulin signals was calculated. The ratios obtained from LacZ-injected mice were normalized to 100%, and the data were given as means ± SD of the percentage of lacZ-transduced controls. *p < 0.05; **p < 0.01; ***p < 0.001 compared with lacZ-transduced mice.