Cell lineage analysis of 5-HT-IR taste cells in buds triple labeled with 5-HT antibodies, β-gal antibodies, and propidium iodide
| Total number cells (N) | Total β-gal-IR cells | β-gal-IR only cells | Double-labeled cells | 5-HT-IR only cells | Total 5-HT-IR cells | p value | |
|---|---|---|---|---|---|---|---|
| TB 1 | 11 | 4 | 0 | 4 | 0 | 4 | 0.01 |
| TB 2 | 9 | 6 | 3 | 3 | 0 | 3 | 0.52 |
| TB 3 | 8 | 3 | 3 | 0 | 2 | 2 | 0.66 |
| TB 4 | 10 | 4 | 3 | 1 | 1 | 2 | 0.99 |
| TB 5 | 13 | 3 | 1 | 2 | 1 | 3 | 0.24 |
| TB 6 | 8 | 5 | 0 | 5 | 0 | 5 | 0.05 |
| TB 7 | 15 | 2 | 0 | 2 | 1 | 3 | 0.03 |
| TB 8 | 16 | 3 | 3 | 0 | 3 | 3 | 0.84 |
| TB 9 | 16 | 2 | 1 | 1 | 2 | 3 | 0.70 |
| TB 10 | 16 | 5 | 3 | 2 | 0 | 2 | 0.17 |
| TB 11 | 20 | 7 | 2 | 5 | 0 | 5 | 0.006 |
| TB 12 | 14 | 5 | 3 | 2 | 0 | 2 | 0.24 |
| TB 13 | 17 | 6 | 2 | 4 | 0 | 4 | 0.02 |
| TB 14 | 24 | 12 | 6 | 6 | 1 | 7 | 0.17 |
| TB 15 | 11 | 5 | 2 | 3 | 0 | 3 | 0.17 |
| TB 16 | 10 | 4 | 3 | 1 | 1 | 2 | 0.99 |
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β-gal-IR cells, 5-HT-IR cells, cells double labeled for β-gal and 5-HT, and propidium iodide-labeled cells were counted in single confocal sections of 16 taste buds. The distribution of these counts was compared with that expected if β-gal-IR and 5-HT-IR labeled cells independently of each other (see Materials and Methods). χ2 analysis was used to compare the distributions. The resultant p value for each taste bud is listed in the far right column. A p value of 0.05 or less indicated that the null hypothesis (that the labels are independent of each other) should be rejected at the 95% confidence level. Samples with only one β-gal+ cell were excluded from analysis because such taste buds provide no information about lineage. N was obtained by counting nuclei stained with propidium iodide in a specified region. TB, Taste bud.
