Interaction of tamalin with the C-terminal regions of mGluRs and GABA_B2 in the yeast two-hybrid system. Interaction of the complete C-terminal regions of mGluR2, 3, 4, 6, 7a, and 8 was tested by filter β-galactosidase assay in the yeast two-hybrid system. For mGluR1a and 5, the last seven and five amino acid C-terminal sequences were used, respectively, because the complete C-terminal sequences of both receptors displayed false positive data without addition of a prey plasmid. ct-GABA_B2 contained the last 80 amino acid sequence of the C terminus of GABA_B2. Positive interaction is indicated as +, and negative is indicated as −.