Activation of c-Jun phosphorylation occurs in the degenerative striatal region. P-c-Jun immunoreactivity was performed using an anti-P-c-Jun antibody. Note a strong induction at3-NP-5 days and 3-NP-6 days treatment. Coimmunostaining with TUNEL indicates that P-c-Jun immunoreactivity occurs in the degenerative region. Note the double labeling between P-c-Jun and TUNEL staining in one striatal neuron (bottom right panel, arrow).