Anoxia–hypoglycemia induced formation of filopodia. A, Time sequence illustrating the formation and modifications of a filopodia induced after a short (10 min) anoxia–hypoglycemia. Scale bar, 5 μm. B, Quantitative analysis of the number of filopodia per 100 μm dendritic length observed over periods of 80 min of observation under control conditions (white column), after a short anoxia–hypoglycemia (black column), after an anoxia–hypoglycemia but with 10 mm BAPTA in the pipette solution (gray column), or a medium containing low calcium (0.1 mm), high magnesium (10 mm), or MK-801 (40 μm; dashed column). Data are mean ± SEM of 11–24 experiments. *p < 0.05.C, Time interval at which filopodia were seen to appear after anoxia–hypoglycemia. Data are expressed as percentage of the total number of events.