Primary microglia, but not neurons, express annexin II, a potential tPA receptor. a, RT-PCR results from microglia (mg) or neurons (n), amplified using either annexin II (ann II) or APP primers. The quality of the neuronal cDNA was established using the APP as a positive control (lane 4). b, Western blot confirming that primary microglia but not neurons express annexin II. The cell lysates used for the Western blot were also probed with the neuronal marker microtubule-associated protein 2 and the microglial marker F4/80 to confirm that the respective cell types had been highly enriched as intended and indicated (data not shown). c, An anti-actin antibody was used to ascertain equal protein loading for the lysates analyzed on the Western blot.