Calcium waves invade the nucleus. A confocal fluorescent image of a cell loaded with 50 μm Oregon Green BAPTA-1 is shown on the left of a. The nucleus can be seen as a bright area in the soma. Thevertical red line through the cell indicates the line of interest used in subsequent line scan time series. The baseline-subtracted time series (ΔF) displayed in pseudocolor after focal application of carbachol (20 μm) is shown on the right. Thevertical white line indicates the time of the first detectable rise in calcium. Dashed white lines mark the nucleus. b, Rises in calcium, plotted as ΔF/F, measured over the dendritic (D), somatic (S), and nuclear (N) regions indicated in aare shown over time. Note the latency between the initiation of the calcium wave and its subsequent nuclear invasion.