BDNF decreases respiratory frequency in vitro. A, Intracellular recordings from a PBC neuron (top traces) recorded simultaneously with integrated population activity (bottom traces) in control (A1) and in the presence of 100 ng/ml BDNF (B1). A2, Bar histograms showing the mean period of rhythmic activity in the presence of either BDNF or NGF and their respective control (CTL) (BDNF control, white bar; n = 12), 100 ng/ml BDNF (black bar; n = 12), NGF control (light gray bar; n = 6), and 100 ng/ml NGF (dark gray bar, n = 6). B2, Bar histograms depicting the mean period of rhythmic activity (n = 6) in control conditions (white bar), 200 nm K252a (gray bar), and 200 nm K252a plus 100 ng/ml BDNF (black bar). BDNF effects are abolished in the presence of K252a. ^*p < 0.05.