ACh-elicited currents in SNc/VTA DA neurons. Effect of αCtxMII. Representative traces of ACh-elicited currents in DA neurons from α6+/+, α4-/-, α6-/-, and α4-/-α6-/- mice. Mean αCtxMII (100 nm) inhibition values are shown in Table 2. In α4-/-α6-/- mice, or inα4-/- animals after αCtxMII application, residual current exhibited the kinetic (fast rise and decay) and pharmacological characteristics (complete inhibition by 1 nm MLA) of α7 nAChR-mediated currents.