Kinesin is selectively reduced and redistributed in T40-AV-infected astrocytes. Double-label immunofluorescence of kinesin heavy chain and Glu-MTs before T40-AV infection (A) and of kinesin and tau 8 hr after T40-AV infection (B). Kinesin staining at high magnification showed vesicular or linear staining distributed over the entire cytoplasm extending to the edge of the cell. This distribution was not identical to that of MTs, although kinesin staining frequently existed in close proximity to MTs in the non-infected cells (A). T40-AV-infected astrocytes showed kinesin staining clustered near the nucleus (B). Western blot analysis of kinesin heavy chain and dynein in the cytoskeletal fractions (C) of T40-AV-infected astrocytes showed a decrease in kinesin with longer infection time, whereas the amount of dynein remained unchanged. Scale bar, 10 μm.