Increased levels of the interferon-inducible subunits of the immunoproteasomes LMP2 and LMP7 in cortex and striatum of HD94 mice. A, Western blot analysis of brain cortical extracts of HD94 mice (HD94) and control littermates (C) with the MCP antibody (raised against native 20 S proteasome), the α1-α7 antibody (that recognizes six of the seven α-subunits of the 20 S proteasome), and the C2 (α6) antibody (raised against the C2 COOH-terminal region and that is able to discriminate the active and latent forms of the proteasome). Membranes are probed with an anti-tubulin antibody to correct for any possible deviation on protein loading. B, Western blot analysis of brain cortical (Cx) and striatal (St) extracts from 14-month-old HD94 mice and control littermates with antibodies against the LMP2 and LMP7 inducible subunits of the immunoproteasome. Membranes are probed with an anti-tubulin antibody to correct for any possible deviation on protein loading. C, Histograms showing the densitometric quantification of LMP2 and LMP7 levels in HD94 versus control samples in 3-, 7-, and 14-month-old mice (^*p < 0.01).