Inhibition of glutamate transporters reduces electrically evoked IPSCs. A, Evoked IPSC recorded from a CA1 pyramidal cell (V_hold = −60 mV) in control solution (gray) and in the presence of the GABA_AR antagonist SR95531 (black).B, Evoked IPSCs in control solution (gray) and in the presence of 300 μm THA (black), a nonselective glutamate transporter antagonist. C, Normalized IPSC amplitudes [Amp. (norm)] during experiments testing the effects of THA (n = 16 cells) with stimulation either continued (filled circles) or suspended (open circles) during wash-in. Error bars indicate SEM.D, IPSC in control solution (gray) and in the presence of 30 μm TBOA (black), a nonselective, nonsubstrate inhibitor of glutamate uptake. E, IPSC in control solution (gray) and in the presence of 300 μm THA (black) after inhibition of GABA synthesis with 250 μm MPA, a GAD inhibitor.F, IPSC in control solution (gray) and in the presence of 300 μm THA (black) after inhibition of astrocytic glutamine synthetase with 1.5 mm MSO.G, IPSCs recorded from a CA1 pyramidal cell after stimulation in stratum radiatum (s. radiatum) in control solution (gray) and in the presence of 300 μm THA (black). H, Summary of effects of glutamate transporter antagonists on IPSC amplitude in various conditions. White numbers in barsindicate the numbers of cells tested. Error bars indicate SD.Asterisks denote a statistically significant difference compared with control (p < 0.05).s. rad., Stratum radiatum.