Formation of higher-order branches in ddaC, ddaD, and ddaE in the larva. A, B, ddaD and ddaE in NP2225/mCD8:: GFP.C–F, ddaC in NP7028 GFP[S65T]/GFP[S65T]. Indicated are formation or elongation of lateral branches (double arrowheads), pruning or retraction of branches (arrows), and a dendrodendritic contact (single arrowhead in D). A, B, Two images 15 hr apart starting at 24–25 hr AEL. C–E, Three time-lapse images taken at 23–24 hr AEL, 39.5–40.5 hr AEL, and 63.5–64.5 hr AEL, respectively. E, Dendritic tiling between ddaC and its contralateral homolog and between ddaC and v′ada in the ventral prime cluster are indicated (top bracket and bottom brackets, respectively). F, 15 min recordings of dendritic terminals in a third-instar larva. Scale bar:A–C, F, 20 μm; D, E, 50 μm.