Responses of ddaC and ddaE to severing branches.A, B, NP7028 GFP[S65T]/GFP[S65T]. C,D, IG1–1 GFP[S65T] mCD8:: GFP/+. Dendrite shafts (arrows) proximal to cell bodies of ddaC (A, 40–42 hr AEL) and of ddaE (C, 20–22 hr AEL) were severed and observed 2 d later (B and D, respectively). In response to severing of the branches, other isoneuronal branches of ddaC filled extensively in the voided field (arrowheads in A and B and bracket in B), but those of ddaE did not (arrowheads inC and D). In B, another DA neuron, perhaps ddaB, was also weakly labeled. A terminal of posterior adjacent ddaC was indicated (double arrowheads in B).D, ddaE before the severing was shown with the same magnification (inset). Scale bar: A, 50 μm; B, 100 μm; C, D, 20 μm.