GFP-tagged NF-L protein aggregates colocalize with ubiquitin and heat shock proteins. A–C, Confocal microscopy showing similar anti-ubiquitin reactivity (B) in untransfected cell and two cells transfected with pGFP/NF–3′UTR. D–F, Aggregates of GFP-tagged NF-L protein (D) in cells transfected with pGFP/NF+3′UTR colocalize with anti-ubiquitin (E) and alter the tincture of the merged image (F). G–I, Aggregates of GFP-tagged NF-L protein (G) colocalize with anti-HSP70 in cells cotransfected with pGFP/NF+3′UTR and pHM6/HSP70 (H, I). J–L, Aggregates of GFP-tagged NF-L protein (J) also colocalize with DsRed-tagged HSP40 in cells cotransfected with pGFP/NF+3′UTR and pDsRed1/HSP40 (K, L). Scale bars, 10 μm.