Effect of intrathecal tertiapin in wild-type mice. Tail-flick latencies of wild-type mice were measured at 49°C (A), 52.5°C (B), and 55°C (C) before and after the intrathecal injection of saline or tertiapin (10, 30, and 100 pmol). The difference between preinjection and postinjection tail-flick latency (ΔTF) was calculated and plotted as a function of tertiapin dose. Separate cohorts of mice were used for each tertiapin dose, and groups (26–29 mice each) were balanced with respect to gender. Tertiapin produced dose-dependent decreases in tail-flick latencies at all three temperatures. *p < 0.05, tertiapin versus saline.