Pharmacological properties of SB 206553 and SB 242084 in CHO-1C7 cells. Cells, prelabeled with [³H]-AA and myo-[³H]inositol, were incubated with the indicated drugs for 25 min in the presence of LiCL (20 mm) and 0.1% fatty acid-free BSA. a–c, Concentration–response curves for SB 242084 and SB 206553 measuring IP accumulation [SB 206553 alone and in the presence of 100 nm SB 242084 (a)], AA release (b), or [γ³⁵S]GTP binding (c). Basal IP accumulation, AA release, and [γ³⁵S]GTP binding levels were as follows: 1544±167 dpm, 5378±789 dpm, and 72±13 pmol/mg protein, respectively. Data are expressed as the mean ± SEM of six (a, b) or four (c) independent experiments.