Zn²⁺ chelation potentiates the ASIC current in neurons from ASIC2-/- mice but not in neurons from ASIC1-/- mice. A, B, Representative traces and summary data showing the lack of potentiation of ASIC current by TPEN in neurons from ASIC1-/- mice; n = 7. C, D, Knock-out of the ASIC2 subunit does not affect TPEN potentiation of ASIC current in cortical neurons; n = 7; ^**p < 0.01. E, F, ASIC currents in neurons from ASIC2-/- mice are inhibited by buffered Zn²⁺ with an average IC₅₀ value of 4.95 ± 0.58 nm; n = 6. G, H, Zn²⁺ chelation also potentiates the ASIC current in cortical neurons from C57BL/6 mice, a wild-type control for ASIC knock-out mice; n = 5; ^*p < 0.05.