NPY attenuates eEPSPs in hypocretin neurons. eEPSPs were recorded under current-clamp at potentials near -70 mV with 30 μm BIC in the bath. They were evoked by electrical stimulation (arrow) in LH, medial or lateral to the recorded cell. A, Typical representative traces showing the NPY actions on the amplitude of eEPSCs. B, The eEPSPs were completely blocked by bath application of AP-5 (50 μm) and CNQX (10 μm), suggesting that they were attributable to the activation of glutamate NMDA and/or AMPA receptor subtypes. C, Bar graph showing the mean effect of NPY (1 μm) on the time-voltage intergral (time × ΔV) of eEPSPs (32.3 ± 4.9%; ^*p < 0.01; n = 8).