Colocalization of mGluR5a with clathrin and mGluR1a in HEK 293 cells. a, Representative laser scanning confocal micrograph showing the colocalization (yellow) of FLAG-mGluR5a labeled with Alexa Fluor 555-conjugated monoclonal FLAG antibody (red) with GFP-clathrin (green). Cell surface-labeled receptors were allowed to endocytose for 20 min at 37°C. b, Representative laser scanning confocal micrograph showing the colocalization (yellow) of FLAG-mGluR1a labeled with Alexa Fluor 555-conjugated monoclonal FLAG antibody (red) with mGluR5a-GFP (green). Cell surface-labeled receptors were allowed to endocytose for 20 min at 37°C. Scale bars, 10 μm.