¹²⁵I-MPPI binding for 5-HT_1A receptors in the hypothalamic Ad-1AP-antisense-injected SERT normal mice. A, Photography of 5-HT_1A binding, GFP expression, and Nissl staining of hypothalamic sections that received Ad-track and Ad-1AP-antisense adenovirus. GFP and Nissl staining sections were adjacent sections with ¹²⁵I-MPPI binding of Ad-1AP-antisense. The black box indicates the area presented in the photograph of GFP expression. The white box represents the area in the Nissl staining. 3V, Third ventricle; AH, anterior hypothalamus; DMN, dorsomedial hypothalamus; LH, lateral hypothalamus; PVN, paraventricular hypothalamus; VMN, ventromedial hypothalamus. B, ¹²⁵I-MPPI binding in the hypothalamus of Ad-1AP-antisense- and Ad-track-injected mice. Data are presented as mean ± SEM for 14-22 mice. One-way ANOVA for AH: F_(1,34) = 4.8, p = 0.03; for PVN: F_(1,34) = 8.0, p = 0.008; for DMN: F_(1,34) = 9.5, p = 0.004; for VMN: F_(1,34) = 6.0, p = 0.02; for LH: F_(1,34) = 0.13, p = 0.58. ^*Significant difference from Ad-track-injected mice (p < 0.05) by Student-Newman-Keuls post hoc test.