PLA₂ is not required for muscarinic modulation of I_Ca. SCG neurons were treated overnight with PTX and studied in perforated-patch configuration. Voltage protocols are as in Figure 2, and applications of 0.1% DMSO, OPC (10 μm), oxo (10 μm), or CdCl₂ (Cd²⁺; 100 μm) are indicated by bars. A, Vehicle control; B, OPC. C, Bars show the inhibition by oxo in control cells or in those treated with OPC. ^**p ≤ 0.01.