Bradykinin (Brady) does not inhibit I_Ca unless PI4-kinase, IP₃-mediated Ca²⁺ release or NCS-1 activity is blocked. SCG cells were cultured overnight with PTX and studied in perforated-patch configuration. Voltage protocols are as in Figure 2, and application of bradykinin (200 nm), oxo (10 μm), and CdCl₂ (Cd²⁺; 100 μm) are indicated by bars. A, Control. B, Wortmannin (Wort; 50 μm; indicated by the bar) was applied 3 min before application of agonists and maintained throughout. C, Xestospongin C (XeC; 1 μm) was applied to neurons 10 min before commencing recording. D, Neurons were shot with cDNA coding for dominant-negative NCS-1, linked as a fusion protein with EGFP, 1 d before recording with the gene gun. E, Bars show pooled data for bradykinin and muscarinic modulation of I_Ca recorded in control and under the experimental conditions indicated (n = 11, 9, 5, 8). ^***p ≤ 0.001.