Supplemental data

Files in this Data Supplement:

• supplemental material - Model of lipid raft mediated TNFR1 signaling after TBI. In a normal rat brain low levels of TNFR1 are present in lipid rafts and are in complex with TRADD, TRAF1, TRAF2, RIP and cIAP-1 and signals survival. Early after TBI, increased levels of TNFR1 recruit into lipid raft microdomains (.), where they associate with the adaptor protein TRADD, FADD, TRAF2, TRAF1, and cIAP-2. TNFR1 and TRAF1 are polyubiquitinated (Ubq(n) in lipid rafts after TBI, which leads to degradation via the proteasome pathway. In later stages after TBI, RIP and cIAP-1 appear to dissociate from TNFR1 complex by an unknown mechanism, and this complex signals death by activating caspase-8.