Decreased phosphorylation of NR1 at S897 in postmortem brain tissue obtained from the Stanley Brain Bank. A, B, Phosphorylation of NR1 on S897 in frontal cortex of patients with schizophrenia (n = 14), bipolar mood disorder (n = 15), major depression (n = 14), and controls (n = 15) obtained from the Stanley Consortium Brain Bank. The decrease in phosphorylation of NR1 on S897 is specific to patients with schizophrenia. Fifty micrograms of total protein extract were loaded in each lane, and samples were examined blind to the affected status (A) as well as after the ID codes were released (B). Representative immunoblots are shown, probed with S897 NR1 antibody (1:1000), anti-NR1 antibody (1:1000), S831 GluR1 antibody (1:1000), S845 GluR1 antibody (1:1000), S380 PTEN antibody (1:2000), and anti-tubulin (1:1000). In all cases, graphs represent the means and SEs of optical densities reflecting the corrected S897 to total NR1 levels.