Free-radical production in TH neurons of the SNc in UCP2 WT/TG mice. A, B, Representative photomicrographs of in situ ROS production assessed by the oxidation of DHE to ethidium by . Left photomicrographs show a representation of double exposure (A) for ethidium (red) and Hoechst nuclear staining (blue) or triple exposure (B) for ethidium, Hoechst nuclear staining, and TH immunofluorescence (green) in UCP2 WT mice. C, D, Representative findings of ethidium and Hoechst nuclear staining (C) with TH immunofluorescence (D) in UCP2 TG mice. Scale bar: (in C) A-D, 10 μm. E, Quantification of the number of red fluorescent mitochondria within TH cells revealed a significant decrease in UCP2-overexpressing animals compared with their WT controls (n = 4, n = 3, respectively). ^*Significant with respect to WT controls (p < 0.05). Error bars represent SEM.