PrP-res^A568 disaggregation and intracellular trafficking in SN56 cells. Confocal fluorescence microscopy 2-4 h (a, a1, c, c1, e, e1) or 3-4 d (b, d, f, f1) after exposure to PrP-res^A568 aggregates of various strains. a, a1, b, m(Ch) PrP-res^A568. c, c1, d, m(22L)PrP-res^A568. e, e1, f, f1, hPrP-res^A568. PrP-res^A568 of the mouse Chandler and 22L strains was much more readily disaggregated and disseminated throughout the cell bodies and cellular projections than the PrP-res^A568 of the hamster 263K strain. Scale bars, 20 μm. Phase-contrast or differential interference contrast images of the cells are shown in combination with the fluorescence images. Arrows point to initial PrP-res^A568 aggregates bound to cells.