Effect of expression of Rab7 Q67L mutant on PrP-res trafficking. SN56 cells expressing GFP-Rab7 Q67L (Q67L) were infected with m(Ch)PrP-res^A568 (PrP-res). a, Cells with large GFP-Rab7 Q67L-positive endosomes/lysosomes that also accumulated m(Ch)PrP-res^A568 (arrows). b, Large m(Ch)PrP-res^A568 aggregate internalized and trafficked to a GFP-Rab7 Q67L-positive endosome/lysosome (arrow). c, m(Ch)PrP-res^A568 and GFP-Rab7 Q67L distributed and colocalized in neurites (arrow). Data are representative of 41 cells visualized 3 d after infection in four independent experiments. Scale bars, 20 μm.