α-Syn in LDCVs. A, Subcellular fractionation of LDCVs. Cell extract from differentiated SH-SY5Y cells overexpressing α-syn was subjected to a linear sucrose gradient centrifugation, and fractions were collected from the top. High-molecular-weight aggregates are marked with a bracket on the right, and monomeric α-syn is marked with an arrowhead. SgII, Secretogranin II; SPh, synaptophysin. B, Immunoelectron microscopy of LDCV fractions (fractions 8 and 9). Arrows indicate 10 nm of gold particles labeling α-syn. Scale bar, 100 nm.