Induction of Fabp7 and Ngn2 mRNAs by Pax6 at the early stage of cortical development. pCAX/mPax6 expression vector was coelectroporated with pCAX/GFP into the telencephalic vesicle of E11.5 WT embryos, which were cultured for 3, 6, 12, and 18 h. GFP fluorescence, immunostaining of Pax6 protein, and Fabp7 mRNA are shown in whole mount. At 3 h after electroporation when Pax6 protein was just synthesized, Fabp7 mRNA was immediately induced by Pax6 overexpression. Strong and persistent expression of Fabp7 was observed at least 18 h after electroporation. In contrast, Ngn2 expression was weakly induced at 3 h but not well maintained until 18 h. Numbers represent the number of embryos analyzed. Scale bar, 0.5 mm.