FRAP in the auditory and vestibular sensory epithelium. a, Z-projection of the bleached area in a CBX-treated utricular macula. b, Perpendicular reconstruction of a Z-series through the basilar papilla taken before and 5 min after bleaching. The horizontal line indicates the area monitored by FRAP. Scale bar, 20 μm. Sequence of optical sections in the basilar papilla (c) and the utricular macula (d) taken before (prebleach) and immediately after bleaching (t = 0) and when the diffusion equilibrium of calcein is approached (t = 5 min). Fluorescence recovers only in supporting cells but not in hair cells (*). Scale bar, 20 μm.