Pattern of GASP1 immunoreactivity over mouse spinal sensorimotor circuits processing nociceptive inputs. A, GASP1 immunoreactivity is found throughout the spinal cord and is particularly abundant in superficial laminas of the spinal dorsal horn (arrowheads; magnified in panel at the right) and in spinal motor neurons (arrow). B, Immunoreactivity for CB1 is also found throughout the spinal dorsal horn and is also dense in the most superficial lamina. C, Dual-immunofluorescence experiments using a rabbit anti-GASP1 antibody and a goat anti-CB1 antibody on sections of DRG reveal a high degree of colocalization between endogenous GASP1 and native CB1. D, Immunofluorescence labeling of DRG sections shows that GASP1 as well as CB1 are widely expressed in the DRG, including nonpeptidergic nociceptive neurons that are identified via staining with TRITC-labeled IB4. Scale bars: A, B, 200 μm; C, D, 40 μm.
