Cannabinoids depress GABAergic transmission in MCH neurons. A, Voltage-clamp recording showing the depressing actions of the CB1R agonist WIN55,212,2 on sIPSC in hypothalamic MCH neurons. These recordings were performed with a KCl-based internal solution, and at a −60 mV holding potential, inhibitory currents were detected as inward currents. B, This graph shows the time course of WIN55,212,2 actions on the normalized frequency of IPSCs in 13 MCH neurons tested. C, D, Graph depicting population WIN55,212,2 actions on frequency of IPSCs with normal ACSF as the external solution (C) or in the presence of AM251 in the bath (D). E, Bar graphs summarizing WIN55,212,2 actions on IPSC frequency with or without AM251 in the external solution. *p < 0.05, statistically significant. Error bars indicate SEM. Win, WIN55,212,2; Ctrl, control.