Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs
- Shiaoching Gong1,
- Martin Doughty1,
- Carroll R. Harbaugh4,
- Alexander Cummins4,
- Mary E. Hatten2,
- Nathaniel Heintz3, and
- Charles R. Gerfen4
- 1Gene Expression Nervous System Atlas Project,
- 2Laboratory of Developmental Neurobiology, and
- 3Laboratory of Molecular Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, and
- 4Laboratory of Systems Neuroscience, National Institute of Mental Health, Bethesda, Maryland 20892
- Correspondence should be addressed to Charles R. Gerfen, Building 111, National Institutes of Health Animal Center, 16701 Elmer School Road, Dickerson, MD 20842. gerfenc{at}mail.nih.gov
Abstract
Transgenic mouse lines are characterized with Cre recombinase driven by promoters of CNS-specific genes using bacterial artificial chromosome (BAC) constructs. BAC-Cre constructs for 10 genes (Chat, Th, Slc6a4, Slc6a2, Etv1, Ntsr1, Drd2, Drd1, Pcp2, and Cmtm5) produced 14 lines with Cre expression in specific neuronal and glial populations in the brain. These Cre driver lines add functional utility to the >500 BAC-EGFP (enhanced green fluorescent protein) transgenic mouse lines that are part of the Gene Expression Nervous System Atlas Project.
- Copyright © 2007 Society for Neuroscience 0270-6474/07/279817-07$15.00/0
