Average (±SEM) number and length (in minutes) of bouts of wake, non-REM and REM sleep, and transitions between the sleep–wake states during the 12 h day and night periods
| Group | Number of bouts | Length of bouts | ||||||
|---|---|---|---|---|---|---|---|---|
| No lesion | BF+LC | BF+TMN+LC | Correlation: number of neurons vs number of bouts | No lesion | BF+LC | BF+TMN+LC | Correlation: number of neurons vs length of bouts | |
| Day | ||||||||
| Wake | 142.3 (5.9) | 90.3 (4.3)* | 82.5 (3.7)* | 0.910*** | 1.6 (0.2) | 2.5 (0.2)** | 2.9 (0.3)* | −0.635# |
| Non-REM | 141.6 (5.9) | 94.8 (2.7)* | 84.2 (4.3)* | 0.905*** | 2.8 (0.1) | 4.4 (0.4)* | 4.9 (0.2)* | −0.779# |
| REM sleep | 77.8 (4.5) | 45.1 (4.6)* | 37.0 (4.9)* | 0.814*** | 1.5 (0.05) | 1.7 (0.1) | 1.7 (0.2) | −0.366 |
| Transitions | 361.7 (14.6) | 230.1 (8.9)* | 203.7 (8.0)* | 0.918*** | ||||
| Night | ||||||||
| Wake | 62.9 (9.0) | 43.9 (6.2) | 40.6 (6.7) | 0.495 | 10.4 (1.9) | 13.8 (2.3) | 16.6 (5.6) | −0.297 |
| Non-REM | 62.2 (8.9) | 45.7 (6.3) | 43.8 (7.3) | 0.424 | 2.1 (0.1) | 3.5 (0.2)* | 4.3 (0.3)*0 | −0.835*** |
| REM sleep | 30.7 (3.7) | 24.1 (4.4) | 22.6 (3.9) | 0.334 | 0.9 (0.1) | 1.7 (0.1)* | 1.9 (0.2)* | −0.756*** |
| Transitions | 155.8 (21.3) | 113.8 (16.5) | 107.1 (17.3) | 0.444 | ||||
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The correlation coefficient represents the relationship between individual sleep–wake states and the number of neurons that are cholinergic (in BF), adenosine deaminase-IR (marker of histamine neurons in the TMN), and dopamine β-hydroxylase-IR (marker of noradrenaline neurons in the LC). Correlations were derived from data from all rats (n = 23) in Table 1, and not from group data.
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*p < 0.01 versus nonlesioned; **p < 0.05 versus nonlesioned; ***p < 0.0001, Pearson product-moment correlation; #p < 0.01, Pearson product-moment correlation; 0p < 0.05 versus BF+LC. A one-way ANOVA was used to test the means of the three groups for each state followed by post hoc tests.
