Synaptic and extrasynaptic aggregation of recombinant γ2. A, Dual live immunostaining shows partial colocalization of recombinant γ2 (red) and GAD6 (green). Large clusters of WT or K289M recombinant γ2 (arrowheads) are often apposed to GAD6-immunopositive terminals, but such colocalization rarely occurs for clusters of R43Q recombinant γ2. B, Similarly, ∼70% recombinant γ2 clusters (red) colocalize with α1-immunopositivity (green) in neurons expressing WT or K289M but not R43Q recombinant GABRG2. The R43Q γ2 clusters are often intercalated between α1 clusters. Scale bar, 5 μm. C, Colocalization of GAD6-immunopositive terminals with recombinant γ2 clusters from 14 (WT), 21 (K289M), and 12 (RQ) neurons. D, Colocalization of recombinant γ2-immunopositive clusters with endogenous α1 clusters from 9 (WT), 11 (K289M), and 6 (R43Q) neurons. *p < 0.05; **p < 0.002.