A, B, Nissl-stained sections of the Cg of wild-type (A) and VMAT2 KO (B) mice at P1. Arrows point to pyknotic profiles. C, Number of pyknotic profiles in layers II/III of the cingulate cortex (P1: WT, n = 7; VMAT2 KO, n = 5; P2: WT, n = 4; VMAT2 KO, n = 4; P3: WT, n = 3; VMAT2 KO, n = 3; P4: WT, n = 3; VMAT2 KO, n = 3). *p < 0.05 indicates that the results are statistically different. D, Electron micrograph showing an apoptotic profile in the superficial layers of Cg of a VMAT2 KO at P1. E, Magnification from D. White arrows point to the disruption of the nuclear envelope. Some chromatin is visible in the cytoplasm (black arrow), whereas organelles such as mitochondria are still preserved (*). TUNEL staining (F, I) and activation of caspase-3 (G, J) and capase-9 (H, K) immunoreactivity were performed on coronal sections of wild-type and VMAT2 KO mice at P1. Scale bar: A, B, 50 μm; D, 800 nm; E, 200 nm; F, H, I, K, 200 μm; G, J, 240 μm.