Table 2.
Percentage of presynaptic and postsynaptic elements immunoreactive for CB1-R in the BNST after injection of the anterograde tracer (PHA-L) in the prefrontal cortex and the retrograde tracer (CTb) in the mesencephalon
| Tracers | Profile | Percentage of CB1 positives profiles | No. of particles per μ m ±SEM | Total no. of profiles | Percentage of total particles distribution |
|---|---|---|---|---|---|
| PHA-L | Presynaptic (terminals) | 92.68 | 1.13 + 0.13 | 86 | 73.83 (membrane) 26.17 (cytoplasm) |
| CTb | Synaptic contact (terminals ± dendrites) | 77.15 | 0.74 + 0.05 | 83 | 70.74 (membrane) 29.86 (cytoplasm) |
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The percentage of CB1-R-labeled presynaptic and postsynaptic compartments relative to PHA-L and postsynaptic CTb is shown. Note that the vast majority of infralimbic terminals within the BNST have the CB1-R. BNST sections used for counting were obtained from four rats. Electron micrographs were taken at a final magnification of 15,000×.
