Marciane Milanski; Giovanna Degasperi; Andressa Coope; Joseane Morari; Raphael Denis; Dennys E. Cintra; Daniela M. L. Tsukumo; Gabriel Anhe; Maria E. Amaral; Hilton K. Takahashi; Rui Curi; Helena C. Oliveira; José B. C. Carvalheira; Silvana Bordin; Mário J. Saad; Lício A. Velloso

Saturated Fatty Acids Produce an Inflammatory Response Predominantly through the Activation of TLR4 Signaling in Hypothalamus: Implications for the Pathogenesis of Obesity

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Figure 3.

A, Immunoblot (IB) analysis of the expressions of pJNK, JNK, pPERK, PERK, GRP78, and peIF2α in hypothalamic protein extracts obtained from rats treated intracerebroventricularly with diluent (DL) or arachidic acid (C20:0) for 1–3 d; some rats were intracerebroventricularly cannulated but received no treatment (−). B, Immunoprecipitation (IP) analysis of the associations of TLR2 and TLR4 with MyD88 in hypothalamic protein extracts obtained from nonintracerebroventricular cannulated (CT) rats, and rats treated intracerebroventricularly with DL or C20:0 for 3 d. The depicted blots are representative of five distinct experiments.

The Journal of NeuroscienceSociety for Neuroscience

Saturated Fatty Acids Produce an Inflammatory Response Predominantly through the Activation of TLR4 Signaling in Hypothalamus: Implications for the Pathogenesis of Obesity

The Journal of Neuroscience Society for Neuroscience