A, Immunoprecipitation (IP) analysis of the associations of TLR2 and TLR4 with MyD88 in hypothalamic protein extracts obtained from rats not intracerebroventricularly cannulated (CT), and rats treated intracerebroventricularly with diluent (DL), arachidic acid (C20:0), TLR2 receptor antibody plus arachidic acid (T2rAb+C20:0) or TLR4 receptor antibody plus arachidic acid (T4rAb+C20:0) for 3 d. B, Immunoblot (IB) analysis of expression of pJNK, JNK, pPERK, PERK, GRP78 and peIF2α in hypothalamic protein extracts. C, PCR analysis of spliced/total XBP-1 transcripts in hypothalamic samples. In A and B, the depicted blots are representative of five distinct experiments; in C, n = 5; different letters mean significant differences between groups, p < 0.05.