A, Rats were fed on high-fat diet for 8 weeks. During the last 7 d of the protocol, the rats were intracerebroventricularly treated with saline (HF), TLR2-inhibiting antibody (HF+T2rAb), or TLR4-inhibiting antibody (HF+T4rAb). Body mass change was measured over the 7 d (A). At the end of the experimental period, hypothalami were obtained for real-time PCR analysis of TNF-α (B), IL-1β (C), IL-6 (D), and IL-10 (E) mRNA determination. In all experiments, n = 5; different letters mean significant differences between groups, p < 0.05.