Biochemical properties of TMEM16B. A , Western blot analysis of MBP fusion proteins containing N- and C-terminal TMEM16B protein fragments and total protein lysates of 293-EBNA cells overexpressing TMEM16B with monoclonal antibody TMEM16B-7H10 and polyclonal antibody TMEM16B-303. B , C , Western blot analysis ( B ) of TMEM16B immunoprecipitated from 293-EBNA cell after digestion with EndoH and PNGase F glycosidases and ( C ) of TMEM16B in mouse retina and brain homogenates. D , Immunofluorescence detection of TMEM16B in 293-EBNA cells. E , Western blot analysis of TMEM16B solubilized with Triton X-100 or SDS. P, Insoluble pellet; S, supernatant. Data shown in B–E were generated with TMEM16B-7H10 antibodies.